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KMID : 1147220170180040130
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Journal of Biomedical and Translational Research
2017 Volume.18 No. 4 p.130 ~ p.135
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Reactive oxygen species (ROS) induces distinct gene expression in human fibroblast like synoviocytes (HFLS)
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Kil Kyung-Min
Jung So-Hee
Jeon In-Sook
Cho Byung-Ki
Park Ji-Kang
Park Kyung-Jin
Kim Yong-Min
Choi Joong-Kook
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Abstract
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Adhesive capsulitis of the shoulder is a common cause of pain that occurs during shoulder movement, thereby restricting shoulder rotation in clinical practice. Although most patients respond to pain relief treatment (NSAID or corticosteroids) by improving their range of motion, it remains poorly understood without any definitive treatment algorithm. In addition to immune cells, synoviocytes, chondrocytes and osteoblasts in the joint are known to produce pro-inflammatory mediators such as reactive oxygen species (ROS), inflammatory cytokines and lipid mediators, presumably contributing to the pathogenesis of osteoarthritis (OA) and adhesive capsulitis. Although inflammation and also fibrosis are proposed to be the basic pathological changes of a frozen shoulder, there is a lack of information regarding the downstream targets of the pro-inflammatory ROS signaling pathway in the synoviocytes and also how these ROS targets are modulated at the transcription level by a corticosteroid - dexamethasone. In this study, we used human fibroblast like synoviocytes (HFLS) to characterize the signaling targets of ROS by employing a human DNA microarray tool and studied the role of dexamethasone in this process. Our data suggest that several genes such as FOS, FOSB and NFkBIZ, which are known to be involved in pro- or anti- inflammation response, are modulated at the transcription level by ROS and dexamethasone.
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KEYWORD
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synoviocytes, reactive oxygen species, dexamethasone, adhesive capsulitis, DNA microarray
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